Product Name: KinSub1RRGSE
Product Number: PE-01AJA95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RRGSE
Product Use: For assaying the phosphotransferase activity of Death-associated protein kinase 3 (DAPK3, UniProt ID O43293). The KinSub1RRGSE peptide demonstrated high phosphotransferase activity with Pim1, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RRGSE was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGRGRRGSEYGRGGG
Product Use: For assaying the phosphotransferase activity of Death-associated protein kinase 3 (DAPK3, UniProt ID O43293). The KinSub1RRGSE peptide demonstrated high phosphotransferase activity with Pim1, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RRGSE was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGRGRRGSEYGRGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1557.7 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Pim1
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1557.7 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Pim1
Scientific Background: DAPK3 (also known as ZIP) is a protein-serine/threonine kinase that is a member of the CAMK group of protein kinases in the DAPK family. This kinase is moderate to highly expressed in most tested human tissues. Orthologues of DAPK3 are highly conserved in animals and plants. DAPK3 is activated by phosphorylation at T180, T225, and T265. Homodimers of DAPK3 or heterodimers with ATF4 formed via a leucine zipper domain is required for full enzymatic activity. DAPK3 plays a role in apoptosis. It phosphorylates core histones H3 and H4, and myosin light chain in vitro. DAPK3 interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4.
References
[1] Preuss U, Landsberg G, Scheidtmann KH. Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase. Nucleic Acids Res. 2003 Feb 1;31(3):878-85. PMID: 12560483.
[2] Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol. 1998 Mar;18(3):1642-51. PMID: 9488481.
[1] Preuss U, Landsberg G, Scheidtmann KH. Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase. Nucleic Acids Res. 2003 Feb 1;31(3):878-85. PMID: 12560483.
[2] Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol. 1998 Mar;18(3):1642-51. PMID: 9488481.
© Kinexus Bioinformatics Corporation 2017