Product Name: KinSub1RQDSF
Product Number: PE-01AHW95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RQDSF
Product Use: For assaying the phosphotransferase activity of Protein kinase C gamma type (PKCg, UniProt ID P05129). The KinSub1RQDSF peptide demonstrated very high phosphotransferase activity with PKCg, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RQDSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGLKRQDSFDRFGGG
Product Use: For assaying the phosphotransferase activity of Protein kinase C gamma type (PKCg, UniProt ID P05129). The KinSub1RQDSF peptide demonstrated very high phosphotransferase activity with PKCg, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RQDSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGLKRQDSFDRFGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1595.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PKCg
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1595.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PKCg
Scientific Background: PKCe (PRKCE, PPK-epsilon) is a member of the AGC group of protein-serine/threonine kinases in the PKC family, and Eta subfamily. This kinase is moderate to highly expressed in most tested human tissues except in the brain and spinal cord where it is poorly expressed. PKCe is activated by phosphorylation at T566, T710 and S729. Phosphorylation of S729 also promotes dephosphorylation of T566 and possibly nuclear import. Phosphorylation at S368 induces interaction with 14-3-3 beta. PKCe is involved in many different cellular functions, such as neuron channel activation, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice indicate that this kinase is important for lipopolysaccharide (LPS)-mediated signalling in activated macrophages and may control anxiety-like behavior. PKCe has been linked with the development of Alzheimer's disease, colorectal adenocarcinoma, prostate and thyroid cancers.
© Kinexus Bioinformatics Corporation 2017