Product Name: KinSub1RPLYF
Product Number: PE-01AHU95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RPLYF
Product Use: For assaying the phosphotransferase activity of calcium/calmodulin-dependent protein-serine kinase 2 alpha (CaMK2a, UniProt ID Q9UQM7). The KinSub1RPLYF peptide demonstrated low phosphotransferase activity with CaMK2b, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RPLYF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGRSRPLYFYVRGYR
Product Use: For assaying the phosphotransferase activity of calcium/calmodulin-dependent protein-serine kinase 2 alpha (CaMK2a, UniProt ID Q9UQM7). The KinSub1RPLYF peptide demonstrated low phosphotransferase activity with CaMK2b, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RPLYF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGRSRPLYFYVRGYR
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1926.2 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CaMK2b
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1926.2 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CaMK2b
Scientific Background: CaMK2a is a protein-serine/threonine kinase of the CAMK group and CAMK2 family. It is a calcium/calmodulin-activated protein kinase involved in neurotransmitter release and long term potentiation in neurons. Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state
References
[1] Silva AJ, Paylor R, Wehner JM, Tonegawa S. Impaired spatial learning in alpha-calcium-calmodulin kinase II mutant mice. Science. 1992 Jul 10;257(5067):206-11. PMID: 1321493.
[2] Thiagarajan TC, Piedras-Renteria ES, Tsien RW. alpha- and betaCaMKII. Inverse regulation by neuronal activity and opposing effects on synaptic strength. Neuron. 2002 Dec 19;36(6):1103-14. PMID: 12495625.
[1] Silva AJ, Paylor R, Wehner JM, Tonegawa S. Impaired spatial learning in alpha-calcium-calmodulin kinase II mutant mice. Science. 1992 Jul 10;257(5067):206-11. PMID: 1321493.
[2] Thiagarajan TC, Piedras-Renteria ES, Tsien RW. alpha- and betaCaMKII. Inverse regulation by neuronal activity and opposing effects on synaptic strength. Neuron. 2002 Dec 19;36(6):1103-14. PMID: 12495625.
© Kinexus Bioinformatics Corporation 2017