Product Name: KinSub1RPKSF
Product Number: PE-01AHT95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub1RPKSF

Product Use: For assaying the phosphotransferase activity of Polo-like protein-serine kinase 1 (Plk1, UniProt ID P53350). The KinSub1RPKSF peptide demonstrated medium phosphotransferase activity with Plk1, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub1RPKSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: GGRSRPKSFCGGGGG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1378.5 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: Plk1

Scientific Background: Plk1 is one of several protein kinases that can phosphorylate KinSub1RPKSF. Human Plk1 is a protein-serine/threonine kinase of 603 amino acid length, with a predicted molecular mass of 68,255 Da. It is a member of the Other group of protein kinases in the Plk family. This kinase shows high variability in human tissue distribution with the highest levels detected in colon, kidney, pancreas and testes, and is notably absent in brain and spinal cord. Orthologues of Plk1 are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. Plk1 is activated by phosphorylation at S49, S137, T210, and S326. Plk1 localizes to centrosomes or spindle pole bodies and undergo dramatic subcellular relocation during the cell cycle. Deregulated activities of Plk's often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics (1). Plk's also regulate the function of the Golgi complex. Deregulated expression of human Plk1 is strongly correlated with the development of many types of malignancies, and ectopic expression of Plk1 dominant negative protein leads to rapid cell death (2). Plk1 has been linked with the development of colon and prostate cancers and lung squamous cell carcinomas (LSCC).
References

[1] Nigg EA, Blangy A, Lane HA. Dynamic changes in nuclear architecture during mitosis: on the role of protein phosphorylation in spindle assembly and chromosome segregation. Exp Cell Res. 1996 Dec 15;229(2):174-80. PMID:8986594

[2] Dai W, Cogswell JP. Polo-like kinases and the microtubule organization center: targets for cancer therapies. Prog Cell Cycle Res. 2003;5:327-34. PMID:14593727