Product Name: KinSub1RDLYV
Product Number: PE-01AHL95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RDLYV
Product Use: For assaying the phosphotransferase activity of Discoidin domain-containing receptor 2 (DDR2, UniProt ID Q16832). The KinSub1RDLYV peptide demonstrated high phosphotransferase activity with TXK, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RDLYV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRDLYVKFGTIG
Product Use: For assaying the phosphotransferase activity of Discoidin domain-containing receptor 2 (DDR2, UniProt ID Q16832). The KinSub1RDLYV peptide demonstrated high phosphotransferase activity with TXK, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RDLYV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRDLYVKFGTIG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1594.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1594.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Scientific Background: DDR2 is one of several protein kinases that can phosphorylate KinSub1RDLYV. Human DDR2 is a receptor protein-tyrosine kinase of 855 amino acid length, with a predicted molecular mass of 96,736 Da. It is a member of the TK group of protein kinases in the DDR family. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain where it is poorly expressed. Orthologues of DDR2 are highly conserved in vertebrates and insects. Phosphorylation of DDR2 at Y471 induces interaction with Shc1, whereas phosphorylation at Y740 induces interaction with Nck2 and SHP2. DDR2 isfeatures a distinct extracellular region encompassing a factor VIII-like domain (1) and is thought to be involved in the regulation of cell growth, differentiation, and metabolism. DDR2 plays a role in the regulation of collagen turnover mediated by smooth muscle cells in obstructive diseases of blood vessels and the lung (2). DDR2 has also been linked with the development of lung large cell carcinomas and spondyloepimetaphyseal dysplasia short limb-hand type (semd-sl).
References
[1] Karn T, Holtrich U, Bräuninger A, Böhme B, Wolf G, Rübsamen-Waigmann H, Strebhardt K. Structure, expression and chromosomal mapping of TKT from man and mouse: a new subclass of receptor tyrosine kinases with a factor VIII-like domain. Oncogene. 1993 Dec;8(12):3433-40. PMID: 8247548.
[2] Ferri N, Carragher NO, Raines EW. Role of discoidin domain receptors 1 and 2 in human smooth muscle cell-mediated collagen remodeling: potential implications in atherosclerosis and lymphangioleiomyomatosis. Am J Pathol. 2004 May;164(5):1575-85. PMID: 15111304.
[1] Karn T, Holtrich U, Bräuninger A, Böhme B, Wolf G, Rübsamen-Waigmann H, Strebhardt K. Structure, expression and chromosomal mapping of TKT from man and mouse: a new subclass of receptor tyrosine kinases with a factor VIII-like domain. Oncogene. 1993 Dec;8(12):3433-40. PMID: 8247548.
[2] Ferri N, Carragher NO, Raines EW. Role of discoidin domain receptors 1 and 2 in human smooth muscle cell-mediated collagen remodeling: potential implications in atherosclerosis and lymphangioleiomyomatosis. Am J Pathol. 2004 May;164(5):1575-85. PMID: 15111304.
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