Product Name: KinSub1RDGSV
Product Number: PE-01AHK95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RDGSV
Product Use: For assaying the phosphotransferase activity of Wee1 protein-tyrosine kinase (Wee1, UniProt ID P30291). The KinSub1RDGSV peptide demonstrated medium phosphotransferase activity with PKCg, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RDGSV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGGRDGSVRGSGIE
Product Use: For assaying the phosphotransferase activity of Wee1 protein-tyrosine kinase (Wee1, UniProt ID P30291). The KinSub1RDGSV peptide demonstrated medium phosphotransferase activity with PKCg, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RDGSV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGGRDGSVRGSGIE
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1359.4 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PKCg
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1359.4 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PKCg
Scientific Background: Wee1 is one of several protein kinases that can phosphorylate KinSub1RDGSV. Human Wee1 is a protein-tyrosine/serine/threonine kinase of 646 amino acid length, with a predicted molecular mass of 71,597 Da. It is a member of the Other group of protein kinases in the Wee family. Wee1 is a nuclear protein that catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase (1). Wee1 is highly expressed in testis. Wee1 kinase phosphorylated the p34 (CDC2) -cyclin B complex on Tyr-15 and inactivated the p34 (CDC2)-cyclin B kinase (2).
References
[1] Heald R, McLoughlin M, McKeon F. Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated cdc2 kinase. Cell. 1993 Aug 13;74(3):463-74. PMID: 8348613.
[2] Parker LL, Piwnica-Worms H. Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase. Science. 1992 Sep 25;257(5078):1955-7. PMID: 1384126.
[1] Heald R, McLoughlin M, McKeon F. Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated cdc2 kinase. Cell. 1993 Aug 13;74(3):463-74. PMID: 8348613.
[2] Parker LL, Piwnica-Worms H. Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase. Science. 1992 Sep 25;257(5078):1955-7. PMID: 1384126.
© Kinexus Bioinformatics Corporation 2017