Product Name: KinSub1RDGSP
Product Number: PE-01AHJ95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RDGSP
Product Use: For assaying the phosphotransferase activity of Muscle, skeletal receptor tyrosine-protein kinase (MUSK, UniProt ID O15146). The KinSub1RDGSP peptide demonstrated high phosphotransferase activity with CDK2, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RDGSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRDGSPCGKGIG
Product Use: For assaying the phosphotransferase activity of Muscle, skeletal receptor tyrosine-protein kinase (MUSK, UniProt ID O15146). The KinSub1RDGSP peptide demonstrated high phosphotransferase activity with CDK2, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RDGSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRDGSPCGKGIG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1372.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CDK2
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1372.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CDK2
Scientific Background: Human Musk is a protein-tyrosine kinase of the TK group of protein kinases in the Musk family. This kinase is moderate to highly expressed in most tested human tissues except in the bladder, brain, pericardium, pituitary, spinal cord, trachea and vagina where it is notably low. Orthologues of Musk are highly conserved in vertebrates and insects. MUSK is necessary for neuromuscular junction formation (1). Musk gene expression is highly regulated during neuromuscular junction and it is involved in intercellular communication present on the surface of cells that are activated by specific protein ligands. Musk members play a key role in growth and differentiation of those cell types. Agrin signals through Musk to cluster acetylcholine receptors on the postsynaptic membrane of the neuromuscular junction. Musk interacts with Dok7, which may regulates its phosphotransferase activity. Dok7 is essential for MUSK activation in cultured myotubes. Musk also plays a critical role in the development of normal blood vessels (2). Musk has been linked with the development of lung neuroendocrine carcinomas.
References
[1] Okada K, Inoue A, Okada M, Murata Y, Kakuta S, Jigami T, Kubo S, Shiraishi H, Eguchi K, Motomura M, Akiyama T, Iwakura Y, Higuchi O, Yamanashi Y. The muscle protein Dok-7 is essential for neuromuscular synaptogenesis. Science. 2006 Jun 23;312(5781):1802-5. PMID:16794080
[2] Kim CH, Xiong WC, Mei L. Regulation of MuSK expression by a novel signaling pathway. J Biol Chem. 2003 Oct 3;278(40):38522-7. Epub 2003 Jul 28. PMID: 12885777
[1] Okada K, Inoue A, Okada M, Murata Y, Kakuta S, Jigami T, Kubo S, Shiraishi H, Eguchi K, Motomura M, Akiyama T, Iwakura Y, Higuchi O, Yamanashi Y. The muscle protein Dok-7 is essential for neuromuscular synaptogenesis. Science. 2006 Jun 23;312(5781):1802-5. PMID:16794080
[2] Kim CH, Xiong WC, Mei L. Regulation of MuSK expression by a novel signaling pathway. J Biol Chem. 2003 Oct 3;278(40):38522-7. Epub 2003 Jul 28. PMID: 12885777
© Kinexus Bioinformatics Corporation 2017