Product Name: KinSub1DPDYA
Product Number: PE-01AGZ95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1DPDYA
Product Use: For assaying the phosphotransferase activity of Protein tyrosine kinase 2 beta (Pyk2, UniProt ID Q14289). The KinSub1DPDYA peptide demonstrated high phosphotransferase activity with Blk, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DPDYA was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGGEDPDYAYPGGGG
Product Use: For assaying the phosphotransferase activity of Protein tyrosine kinase 2 beta (Pyk2, UniProt ID Q14289). The KinSub1DPDYA peptide demonstrated high phosphotransferase activity with Blk, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DPDYA was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGGEDPDYAYPGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1447.4 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1447.4 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Scientific Background: Pyk2 is one of several protein kinases that can phosphorylate KinSub1DPDYA. Human Pyk2 (also known as FAK2/RAFTK) is a protein-tyrosine kinase of 1009 amino acid length, with a predicted molecular mass of 115,875 Da. It is a member of the TK group of protein kinases in the Fak family. Unlike FAK, which is widely expressed in various tissues and links transmembrane integrin receptors to intracellular pathways, Pyk2 is expressed mainly in the central nervous system and in cells derived from hematopoietic lineages. Orthologues of Pyk2 are highly conserved in mammals and birds. Pyk2 can be activated by a variety of extracellular signals that elevate intracellular calcium concentration, and by stress signals (1). Pyk2 is activated by phosphorylation at Y402 and Y579. Phosphorylation at Y402 also induces interaction with ErbB2, RasGAP and Src. In osteoclasts, although FAK is expressed, Pyk2 appears to be the predominant mediator of integrin alpha(v)beta3 signalling events that influence osteoclast physiology and pathology (2).
References
[1] Avraham H, Park SY, Schinkmann K, Avraham S. RAFTK/Pyk2-mediated cellular signalling. Cell Signal. 2000 Mar;12(3):123-33. PMID: 10704819
[2] Xiong WC, Feng X. Pyk2 and FAK in osteoclasts. Front Biosci. 2003 Sep 1;8:d1219-26. PMID:12957821
[1] Avraham H, Park SY, Schinkmann K, Avraham S. RAFTK/Pyk2-mediated cellular signalling. Cell Signal. 2000 Mar;12(3):123-33. PMID: 10704819
[2] Xiong WC, Feng X. Pyk2 and FAK in osteoclasts. Front Biosci. 2003 Sep 1;8:d1219-26. PMID:12957821
© Kinexus Bioinformatics Corporation 2017