Product Name: KinSub1DGMYV
Product Number: PE-01AGX95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub1DGMYV

Product Use: For assaying the phosphotransferase activity of Fibroblast growth factor receptor 3 (FGFR3, UniProt ID P22607). The KinSub1DGMYV peptide demonstrated high phosphotransferase activity with Brk, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub1DGMYV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: GGGGDGMYVEPGGGG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1265.3 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: Brk

Scientific Background: FGFR3 is one of several protein kinases that can phosphorylate KinSub1DGMYV. Human FGFR3 is a receptor protein-tyrosine kinase of 806 amino acid length, with a predicted molecular mass of 87,710 Da. It is a member of the TK group of protein kinases in the Fibroblast Growth Factor Receptor family, which includes 4 high-affinity receptors for FGF's 1 to 17. This kinase is moderate to highly expressed in most tested human tissues. Orthologues of FGFR3 are highly conserved in vertebrates and insects. FGFR3 is activated by binding of fibroblast growth factor (FGF), which induces dimerization and transautophosphorylation. Phosphorylation of Y724 and Y760 increases phosphotransferase activity and induces interaction with SH2-B-beta. FGFR3 plays a role in several important cellular processes, including regulation of cell growth and division, determination of cell fate, formation of blood vessels, wound healing and embryo development (1). FGFR3 is involved in the development and maintenance of bone and brain tissue. Mutations in FGFR3 have been implicated in causing bladder cancer, cancer of white blood cells (multiple myeloma) and cervical cancer (2). FGFR3 has also been linked with the development of colorectal cancer as well as dwarfism and facial and other morphogenetic disorders.
References

[1] Chen L, Deng CX. Roles of FGF signaling in skeletal development and human genetic diseases. Front Biosci. 2005 May 1;10:1961-76. PMID: 15769677

[2] Mhawech-Fauceglia P, Cheney RT, Fischer G, Beck A, Herrmann FR. FGFR3 and p53 protein expressions in patients with pTa and pT1 urothelial bladder cancer. Eur J Surg Oncol. 2006 Mar;32(2):231-7. Epub 2006 Jan 18. PMID: 16412606