Product Name: KinSub1DGDSY
Product Number: PE-01AGW95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1DGDSY
Product Use: For assaying the phosphotransferase activity of Src oncogene-encoded protein-tyrosine kinase (Src, UniProt ID P12931). The KinSub1DGDSY peptide demonstrated high phosphotransferase activity with Src, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DGDSY was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGEDGDSYYGWGHG
Product Use: For assaying the phosphotransferase activity of Src oncogene-encoded protein-tyrosine kinase (Src, UniProt ID P12931). The KinSub1DGDSY peptide demonstrated high phosphotransferase activity with Src, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DGDSY was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGEDGDSYYGWGHG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1512.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Src
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1512.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Src
Scientific Background: Src is one of several protein kinases that can phosphorylate KinSub1DGDSY. Human Src is a protein-tyrosine kinase of 536 amino acid length, with a predicted molecular mass of 59835 Da. It is a member of the TK group of protein kinases in the Src family. This kinase is widely distributed from low to moderate levels in most human tissues with highest expression in pancreas and stomach. Orthologues of Src are highly conserved in vertebrates and insects. Src was originally identified as a transforming protein of the Rous sarcoma virus (RSV) that had enzymatic ability to phosphorylate tyrosine in protein substrates (1). Src is activated by phosphorylation at S12, S75, Y419 and T420. S12 phosphorylation promotes interaction with PPP2R2C and Y410 phosphorylation induces interaction with Cbl-c. Phosphorylation at S17 may reduce phosphorylation at S12. Phosphorylation of S97 and Y530 inhibits Src phosphotransferase activity. Src is overexpressed and activated in a large number of human malignancies and has been linked to the development of cancer and progression to distant metastases (2). In addition to increasing cell proliferation, a key role of Src in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumour progression. Src has also been linked with the development of colon cancer.
References
[1] Collett MS, Erikson RL. Protein kinase activity associated with the avian sarcoma virus Src gene product. Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4. PMID: 205879
[2] Jacobs C, Rübsamen H. Expression of pp60c-Src protein kinase in adult and fetal human tissue: high activities in some sarcomas and mammary carcinomas. Cancer Res. 1983 Apr;43(4):1696-702. PMID: 6403227
[1] Collett MS, Erikson RL. Protein kinase activity associated with the avian sarcoma virus Src gene product. Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4. PMID: 205879
[2] Jacobs C, Rübsamen H. Expression of pp60c-Src protein kinase in adult and fetal human tissue: high activities in some sarcomas and mammary carcinomas. Cancer Res. 1983 Apr;43(4):1696-702. PMID: 6403227
© Kinexus Bioinformatics Corporation 2017