Product Name: KinSub1DDLYY
Product Number: PE-01AGT95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1DDLYY
Product Use: For assaying the phosphotransferase activity of B lymphoid tyrosine kinase (Blk, UniProt ID P51451). The KinSub1DDLYY peptide demonstrated very high phosphotransferase activity with Blk, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDLYY was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGGEDDLYYAPGGGG
Product Use: For assaying the phosphotransferase activity of B lymphoid tyrosine kinase (Blk, UniProt ID P51451). The KinSub1DDLYY peptide demonstrated very high phosphotransferase activity with Blk, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDLYY was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGGEDDLYYAPGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1463.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1463.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Scientific Background: Blk is one of several protein kinases that can phosphorylate KinSub1DDLYY. Human Blk (also known as B lymphoid kinase) is a protein-tyrosine kinase of 505 amino acid length, with a predicted molecular mass of 57,706 Da. It is a member of the TK group of protein kinases in the Src family. It has been reported that Blk is expressed specifically in the B cell lineage and plays a role in signal transduction pathway that is restricted to B lymphoid cells (1). However, this kinase has low to reasonably high expression (e.g. skeletal muscle, kidney, thyroid, breast) in most tested human tissues except in the brain and spinal cord where it is poorly expressed. Orthologues of Blk are highly conserved in mammals and birds. Blk is inhibited by phosphorylation at Y501. Blk has been linked with the development of colorectal adenocarcinomas. Stimulation of resting B-lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces activation of Blk (2).
References
[1] Dymecki SM, Niederhuber JE, Desiderio SV. Specific expression of a tyrosine kinase gene, blk, in B lymphoid cells. Science. 1990 Jan 19;247(4940):332-6. PMID: 2404338.
[2] Burkhardt AL, Brunswick M, Bolen JB, Mond JJ. Anti-immunoglobulin stimulation of B lymphocytes activates src-related protein-tyrosine kinases. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7410-4. PMID: 1714601.
[1] Dymecki SM, Niederhuber JE, Desiderio SV. Specific expression of a tyrosine kinase gene, blk, in B lymphoid cells. Science. 1990 Jan 19;247(4940):332-6. PMID: 2404338.
[2] Burkhardt AL, Brunswick M, Bolen JB, Mond JJ. Anti-immunoglobulin stimulation of B lymphocytes activates src-related protein-tyrosine kinases. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7410-4. PMID: 1714601.
© Kinexus Bioinformatics Corporation 2017