Product Name: KinSub1DDLYP
Product Number: PE-01AGR95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1DDLYP
Product Use: For assaying the phosphotransferase activity of Mast/stem cell growth factor receptor protein-tyrosine Kit (Kit, UniProt ID P10721). The KinSub1DDLYP peptide demonstrated very high phosphotransferase activity with Brk, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDLYP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGEDDLYPGPGGGG
Product Use: For assaying the phosphotransferase activity of Mast/stem cell growth factor receptor protein-tyrosine Kit (Kit, UniProt ID P10721). The KinSub1DDLYP peptide demonstrated very high phosphotransferase activity with Brk, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1DDLYP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGGEDDLYPGPGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1303.3 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1303.3 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Scientific Background: Kit (SCFR) is one of several protein kinases that can phosphorylate KinSub1DDLYP. Human Kit is a protein-tyrosine kinase of 976 amino acid length, with a predicted molecular mass of 109,865 Da. It is a member of the TK group of protein kinases in the PDGFR family. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of Kit are highly conserved in vertebrates, including amphibians. Mast,stem cell growth factor (steel factor) binding induces Kit dimerization and autophosphorylation. Protein interactions are induced with phosphorylation at Y568 (with APS, Cbl, SHP2, & Src), Y900 (with Crk & PIK3C2A) and Y936 (with APS, Cbl, Grb2 & Grb7). Phosphorylation of S741 and S746 inhibits the kinase activity of Kit. It was first identified as the cellular homologue of the feline sarcoma viral oncogene v-kit. Mutations in Kit are associated with gastrointestinal stromal tumors (GIST), mast cell leukemia, acute myelogenous leukemia (AML), and piebaldism. Recently, deregulation of the Kit by the prevalent kinase activation loop mutation D816V has served as a focal point in therapeutic strategies aimed curbing neoplastic mast cell growth (2). Kit has also been linked with the development of colorectal adenocarcinomas and melanoma progression.
References
[1] Berger SA. Signaling pathways influencing SLF and c-kit-mediated survival and proliferation. Immunol Res. 2006;35(1-2):1-12. PMID: 17003505
[2] Gotlib J. KIT mutations in mastocytosis and their potential as therapeutic targets. Immunol Allergy Clin North Am. 2006 Aug;26(3):575-92. PMID: 16931294
[1] Berger SA. Signaling pathways influencing SLF and c-kit-mediated survival and proliferation. Immunol Res. 2006;35(1-2):1-12. PMID: 17003505
[2] Gotlib J. KIT mutations in mastocytosis and their potential as therapeutic targets. Immunol Allergy Clin North Am. 2006 Aug;26(3):575-92. PMID: 16931294
© Kinexus Bioinformatics Corporation 2017