Product Name: KinSub1DDDYG
Product Number: PE-01AGK95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub1DDDYG

Product Use: For assaying the phosphotransferase activity of Ephrin type-B receptor 3 protein-tyrosine kinase (EphB3, UniProt ID P54753). The KinSub1DDDYG peptide demonstrated very high phosphotransferase activity with Blk, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub1DDDYG was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: DGGEDDDYGGFGGHG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1453.4 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: Blk

Scientific Background: EphB3 is one of several protein kinases that can phosphorylate KinSub1DDDYG. Human EphB3 is a receptor protein-tyrosine kinase of 998 amino acid length, with a predicted molecular mass of 110,330 Da. It is a member of the TK group of protein kinases in the Eph family. EphB3 is expressed during embryonic development in multiple regions of the central nervous system. In adult brain, EphB3 is expressed in the cerebellum, raphe pallidus, hippocampus, entorhinal cortex, and both motor and sensory cortices (1), and is moderate to highly expressed in most tested adult human tissues. Orthologues of EphB3 are highly conserved in vertebrates, including amphibians. EphB3 is activated by binding ephrin-B1, B2, or B3. Phosphorylation at Y614 induces interaction with Crk, Fyn, and RasGAP. EphB3 is involved in the maintenance of mature neuronal connections and/or re-arrangement of synaptic connections during late stages of development. EphB3 plays a role in the regulation of cell adhesion and migration, and the catalytic activity of EphB3 is required for inhibition of integrin-mediated cell adhesion (2). EphB3 has been linked with the development of lung neuroendocrine carcinomas and lung small cell carcinomas (LSCC).
References

[1] Willson CA, Foster RD, Onifer SM, Whittemore SR, Miranda JD. EphB3 receptor and ligand expression in the adult rat brain. J Mol Histol. 2006 Nov;37(8-9):369-80. Epub 2006 Nov 11. PMID: 17103029

[2] Miao H, Strebhardt K, Pasquale EB, Shen TL, Guan JL, Wang B. The phosphorylation of EphB2 receptor regulates migration and invasion of human glioma cells. J Biol Chem. 2005 Jan 14;280(2):923-32. Epub 2004 Nov 9. PMID:15536074