Product Name: AcCoACarbox (73-85) S77A, KinSub
Product Number: PE-01ADR95
| Size: | 200 µg | Price: | 48.00 | |
| 1 mg | $US | 96.00 | ||
| 5 mg | 210.00 |
Peptide Name: AcCoACarbox (73-85) S77A, KinSub
Product Use: For assaying the phosphotransferase activities of 5'-AMP-activated protein kinase catalytic subunit alpha-1 (UniProt ID Q13131) and 5'-AMP-activated protein kinase, catalytic subunit alpha-2 (AMPK1a2, UniProt ID P54646).
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Based on Acetyl-Coenzyme A carboxylase phosphosite
Peptide Sequence: HMRSAMSGLHLVKRR
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Free carboxyl
Peptide Molecular Mass Calculated: 1336.6 Da
Product Use: For assaying the phosphotransferase activities of 5'-AMP-activated protein kinase catalytic subunit alpha-1 (UniProt ID Q13131) and 5'-AMP-activated protein kinase, catalytic subunit alpha-2 (AMPK1a2, UniProt ID P54646).
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Based on Acetyl-Coenzyme A carboxylase phosphosite
Peptide Sequence: HMRSAMSGLHLVKRR
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Free carboxyl
Peptide Molecular Mass Calculated: 1336.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: AMPK (A1/B1/G1); (A1/B1/G2); (A1/B1/G3); (A1/B2/G1); (A2/B1/G1)
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: AMPK (A1/B1/G1); (A1/B1/G2); (A1/B1/G3); (A1/B2/G1); (A2/B1/G1)
Scientific Background: AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding of AMP to the gamma subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signalling pathways in response to adiponectin, leptin and CAMKK-beta.
References
[1] Hardie DG. The AMP-activated protein kinase pathway--new players upstream and downstream. J Cell Sci. 2004 Nov 1;117(Pt 23):5479-87. PMID: 15509864.
[2] Kahn BB, Alquier T, Carling D, Hardie DG. AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab. 2005 Jan;1(1):15-25. PMID: 16054041.
[1] Hardie DG. The AMP-activated protein kinase pathway--new players upstream and downstream. J Cell Sci. 2004 Nov 1;117(Pt 23):5479-87. PMID: 15509864.
[2] Kahn BB, Alquier T, Carling D, Hardie DG. AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab. 2005 Jan;1(1):15-25. PMID: 16054041.
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