Product Name: RPS6 (230-239) KinSub
Product Number: PE-01ADP95
| Size: | 200 µg | Price: | 32.00 | |
| 1 mg | $US | 64.00 | ||
| 5 mg | 140.00 |
Peptide Name: RPS6 (230-239) KinSub
Product Use: For assaying the phosphotransferase activities of the ribosomal S6 protein kinases RSK1 (S6 kinase alpha 2, UniProt ID Q15418), RSK2 (S6 kinase alpha 3, UniProt ID P51812), RSK3 (S6 kinase alpha 1, UniProt ID Q15349), RSK4 (S6 kinase alpha 6, UniProt ID Q9UK32), MSK1 (S6 kinase alpha 5, UniProt ID O75582), Ribosomal protein S6 kinase 1 (p70S6K, UniProt ID P23443), and Ribosomal protein S6 kinase 2 (p70S6Kb, UniProt ID Q9UBS0).
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Based on ribosomal protein S6 phosphosite
Peptide Sequence: KRRRLSSLRA
Peptide Modifications N Terminus: Free amino
Product Use: For assaying the phosphotransferase activities of the ribosomal S6 protein kinases RSK1 (S6 kinase alpha 2, UniProt ID Q15418), RSK2 (S6 kinase alpha 3, UniProt ID P51812), RSK3 (S6 kinase alpha 1, UniProt ID Q15349), RSK4 (S6 kinase alpha 6, UniProt ID Q9UK32), MSK1 (S6 kinase alpha 5, UniProt ID O75582), Ribosomal protein S6 kinase 1 (p70S6K, UniProt ID P23443), and Ribosomal protein S6 kinase 2 (p70S6Kb, UniProt ID Q9UBS0).
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Based on ribosomal protein S6 phosphosite
Peptide Sequence: KRRRLSSLRA
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Free carboxyl
Peptide Molecular Mass Calculated: 1242.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: RSK1; RSK2; RSK3
Peptide Molecular Mass Calculated: 1242.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: RSK1; RSK2; RSK3
Scientific Background: p70S6K is one of several protein kinases that can phosphorylate RPS6 (230-239). Human p70S6K is a protein-serine/threonine kinase of 525 amino acid length, with a predicted molecular mass of 59,140 Da. It is a member of the AGC group of protein kinases in the RSK family, and p70 subfamily. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord where it is more moderately expressed. Orthologues of p70S6K are highly conserved in animals and plants. p70S6K is activated by phosphorylation at T252 (by PDK1), S394 (by mTOR), T412 (by mTOR), S427, S434 (by ERK1/2), S441, T444 (by ERK1/2) and S447 (by ERK1/2). p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 (1). It is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K phosphotransferase activity changes during the cell cycle, and increases 20-fold in G1 cells released from G0 (2). It has been linked with the development of diet-induced obesity, colon adenocarcinoma and breast cancer.
References
[1] Ferrari S, Thomas G. S6 phosphorylation and the p70s6k/p85s6k. Crit Rev Biochem Mol Biol. 1994;29(6):385-413. PMID: 7705100
[2] Edelmann HM, Kühne C, Petritsch C, Ballou LM. Cell cycle regulation of p70 S6 kinase and p42/p44 mitogen-activated protein kinases in Swiss mouse 3T3 fibroblasts. J Biol Chem. 1996 Jan 12;271(2):963-71. PMID: 8557712
[1] Ferrari S, Thomas G. S6 phosphorylation and the p70s6k/p85s6k. Crit Rev Biochem Mol Biol. 1994;29(6):385-413. PMID: 7705100
[2] Edelmann HM, Kühne C, Petritsch C, Ballou LM. Cell cycle regulation of p70 S6 kinase and p42/p44 mitogen-activated protein kinases in Swiss mouse 3T3 fibroblasts. J Biol Chem. 1996 Jan 12;271(2):963-71. PMID: 8557712
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