Product Name: LRRKtide KinSub
Product Number: PE-01ADD95
Size: 200 µg      Price:48.00
1 mg      $US96.00
5 mg      210.00
Peptide Name: LRRKtide KinSub

Product Use: For assaying the phosphotransferase activities of Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1, UniProt ID Q38SD2) and Leucine-rich repeat kinase 2 (LRRK2, UniProt ID Q5S007).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: Based on common sequence in ezrin, moesin and radixin

Peptide Sequence: RLGRDKYKTLRQIRQ

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Free carboxyl

Peptide Molecular Mass Calculated: 1931.3 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: LRRK2

Scientific Background: LRRK1 and LRRK2 are protein-serine kinases that are members of the TKL group of protein kinases in the LRRK2 family. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of LRRK2 are commonly conserved in vertebrates. Binding of GTP can stimulate its phosphotransferase activity.
References

[1] Nichols RJ, Dzamko N, Hutti JE, Cantley LC, Deak M, Moran J, Bamborough P, Reith AD and Alessi DR (2009) Substrate specificity and inhibitors of LRRK2, a protein kinase mutated in Parkinson's disease. Biochem. J. 424: 47-60. doi:10.1042/BJ20091035

[2] Jaleel M, Nichols RJ, Deak M, Campbell DG, Gillardon F, Knebel A and Alessi DR (2007) LRRK2 phosphorylates moesin at threonine-558: characterization of how Parkinson’s disease mutants affect kinase activity Biochem. J. 405, 307–317; doi:10.1042/BJ20070209