Product Name: Mouse Pancreas
Product Number: LW6221S
Size: | 200 µg | | Price: | 69.00 |
| | | $US | |
Full Name: Mouse pancreas - mixed gender - Whole lysate
Product Type Specific: Whole lysate in SDS-PAGE sample buffer
Product Description: Mouse pancreas - mixed gender - Whole lysate boiled in SDS-PAGE sample buffer
Product Method: Lysates were prepared from tissues that were homogenized by sonication in buffer formulated with 1% Triton X-100, 60 mM ß-glycerophosphate, pH 7.2, 20 mM MOPS, 20 mM sodium pyrophosphate, 30 mM sodium fluoride, 5 mM EDTA, 3 mM benzamidine, 2 mM EGTA, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonylfluoride, 1 mM dithiothreitol, 10 µM leupeptin, and 5 µM pepstatin. Lysates were prepared following sonication and 30 min ultracentrifugation at 100,000 rpm. Lysates were further diluted in SDS-PAGE sample buffer to a final concentration of 2 mg/ml.
Storage Conditions: -20°C