Full Name: Human epidermoid carcinoma A431 cells - Whole lysate

Product Type Specific: Whole lysate

Product Description: Whole lysate from A431 cells that were cultivated to 70-90% confluency and deprived of serum for 18-20 hours and then treated for 100 ng/ml epidermal growth factor (EGF) for 5 minutes prior to harvesting

Product Method: A431 cells were cultivated to 70-90% confluency and deprived of serum for 18-20 hours and then treated for 100 ng/ml epidermal growth factor (EGF) for 5 minutes prior to harvesting. Lysates were prepared from scrapped cells that were homogenized by sonication in buffer formulated with 1% Triton X-100, 60 mM ß-glycerophosphate, pH 7.2, 20 mM MOPS, 20 mM sodium pyrophosphate, 30 mM sodium fluoride, 5 mM EDTA, 3 mM benzamidine, 2 mM EGTA, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonylfluoride, 1 mM dithiothreitol, 10 µM leupeptin, and 5 µM pepstatin A. Lysates were prepared following sonication and 30 min ultracentrifugation at 100,000 rpm. Lysates were further diluted in homogenizing buffer at a final concentration of 3 mg/ml. Lysates were further diluted in homogenizing buffer at a final concentration of 3 mg/ml.

Storage Conditions: -70°C

Lysate Use Description: For testing antibodies by immunoprecipitation or immunoblotting, and for assays of enzymes.

Related Product 1: Cat. No.: LW0103S - A431 whole lysates from EGF-treated cells (2 mg/ml) in SDS-PAGE sample buffer

Related Product 2: Cat. No.: LW0101F - A431 whole lysates from untreated cells (3 mg/ml)

Related Product 3: Cat. No.: LW0101S - A431 whole lysates from untreated cells (2 mg/ml) in SDS-PAGE sample buffer