Product Name: PCYT1B-pS315+pS319
Product Number: AB-PN546
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: CTP: phosphocholine cytidylyltransferase isoform B
Target Alias: CCT B; CCT-beta; CCTB; Choline-phosphate cytidylyltransferase B; CT B; CTB; CTP:phosphocholine cytidylyltransferase B; PCY1B; PCYT1B; phosphate cytidylyltransferase 1, choline, beta; Phosphorylcholine transferase B
Product Type Specific: PCYT1B phosphosite-specific antibody
Antibody Code: PN546
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S315+S319
Protein UniProt: Q9Y5K3
Protein SigNET: Q9Y5K3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: CCT B; CCT-beta; CCTB; Choline-phosphate cytidylyltransferase B; CT B; CTB; CTP:phosphocholine cytidylyltransferase B; PCY1B; PCYT1B; phosphate cytidylyltransferase 1, choline, beta; Phosphorylcholine transferase B
Product Type Specific: PCYT1B phosphosite-specific antibody
Antibody Code: PN546
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S315+S319
Protein UniProt: Q9Y5K3
Protein SigNET: Q9Y5K3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCYT1B; CCTB sequence peptide Cat. No.: PE-04ARF95
Antibody Immunogen Sequence: LQAL(pS)PKQ(pS)PV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L311 to V321; Near the C-terminus of the protein. This is the main in vivo phosphorylation site in PCYT1B.
Antibody Immunogen Sequence: LQAL(pS)PKQ(pS)PV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L311 to V321; Near the C-terminus of the protein. This is the main in vivo phosphorylation site in PCYT1B.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in A431, HeLa and MCF7 cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-52 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in A431, HeLa and MCF7 cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-52 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HeLa and MCF7 cells.
Related Product 1: PCYT1B-pS315+pS319 blocking peptide
Related Product 2: PCYT1A-pS329+pS331 phosphosite-specific antibody (Cat. No.: AB-PN561)
Related Product 3: PCYT1A-pT342+pS343 phosphosite-specific antibody (Cat. No.: AB-PN547)
Related Product 4: PCYT1A-pY359+pS362 phosphosite-specific antibody (Cat. No.: AB-PN548)
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HeLa and MCF7 cells.
Related Product 1: PCYT1B-pS315+pS319 blocking peptide
Related Product 2: PCYT1A-pS329+pS331 phosphosite-specific antibody (Cat. No.: AB-PN561)
Related Product 3: PCYT1A-pT342+pS343 phosphosite-specific antibody (Cat. No.: AB-PN547)
Related Product 4: PCYT1A-pY359+pS362 phosphosite-specific antibody (Cat. No.: AB-PN548)
Scientific Background: PCYT1B is the rate limiting enzyme in the biosynthetic pathway for phosphatidylcholine. It catalyzes the reaction: CTP + phosphocholine = diphosphate + CDP-choline. It is an ambiquitous enzyme that resides in the cytoplasm of cells in an inactive form. Diacylglycerol, acidic phospholipids and fatty acids drive the binding of PCYT1B to the endoplasmic reticulum and stimulation of its catalytic activity.
Figure 1. Epitope mapping of PCYT1B-pS315+pS319 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to PCYT1B-pS315+pS319.
© Kinexus Bioinformatics Corporation 2017