Product Name: TGM2-pY369
Product Number: AB-PN541
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Protein-glutamine gamma-glutamyltransferase 2
Target Alias: C polypeptide; G-ALPHA-h; GNAH; Protein-glutamine gamma-glutamyltransferase 2; protein-glutamine-gamma-glutamyltransferase; TG(C); TG2; TGase C; TGase H; TGase-2; TGase-H; TGC; TGM2; Tissue transglutaminase; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase C; Transglutaminase H; Transglutaminase-2
Product Type Specific: TGM2 phosphosite-specific antibody
Antibody Code: PN541
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y369
Protein UniProt: P21980
Protein SigNET: P21980
Target Alias: C polypeptide; G-ALPHA-h; GNAH; Protein-glutamine gamma-glutamyltransferase 2; protein-glutamine-gamma-glutamyltransferase; TG(C); TG2; TGase C; TGase H; TGase-2; TGase-H; TGC; TGM2; Tissue transglutaminase; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase C; Transglutaminase H; Transglutaminase-2
Product Type Specific: TGM2 phosphosite-specific antibody
Antibody Code: PN541
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y369
Protein UniProt: P21980
Protein SigNET: P21980
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TGM2 sequence peptide Cat. No.: PE-04ADQ95
Antibody Immunogen Sequence: EGT(pY)CCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E366 to G372; In the region between the Transglut_core and Transglut_C domains. This is the major in vivo phosphorylation site in TGM2.
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TGM2 sequence peptide Cat. No.: PE-04ADQ95
Antibody Immunogen Sequence: EGT(pY)CCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E366 to G372; In the region between the Transglut_core and Transglut_C domains. This is the major in vivo phosphorylation site in TGM2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in HepG2 cells, and weak detection in Jurkat cells.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in HepG2 cells, and weak detection in Jurkat cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 82-92 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: In HepG2 cells, insulin decreases 75 and 58 kDa proteins. Almost no significant cross-reactivities were detected in Jurkat and T98G cells.
Related Product 1: TGM2-pY369 blocking peptide
Antibody Specificity: High
Antibody Cross Reactivity: In HepG2 cells, insulin decreases 75 and 58 kDa proteins. Almost no significant cross-reactivities were detected in Jurkat and T98G cells.
Related Product 1: TGM2-pY369 blocking peptide
Scientific Background: TGM2 is a member of the transglutaminase family of enzymes that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. The protein acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis.
Figure 1. Epitope mapping of TGM2-pY369 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to TGM2-pY369.
© Kinexus Bioinformatics Corporation 2017