Target Full Name: TBC1 domain family member 7

Target Alias: Cell migration-inducing protein 23; dJ257A7.3; DKFZp686N2317; FLJ32666; PIG51; TBC1 domain family member 7; TBC1 domain family, member 7; TBC1D7; TBC7; TBCD7

Product Type Specific: TBC1D7 phosphosite-specific antibody

Antibody Code: PN540

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y14

Protein UniProt: Q9P0N9

Protein SigNET: Q9P0N9

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human TBC1D7 sequence peptide Cat. No.: PE-04AEE99

Antibody Immunogen Sequence: SVY(pY)EKV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues S11 to V17; In the N-terminus of protein. This is the major in vivo phosphorylation site for the protein.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.