Target Full Name: Signaling threshold-regulating transmembrane adapter 1

Target Alias: gp30/40; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SHP2 interacting transmembrane adaptor; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; signaling threshold regulating transmembrane adaptor 1; Signaling threshold-regulating transmembrane adapter 1; SIT; SIT1; suppression inducing transmembrane adaptor 1; Suppression-inducing transmembrane adapter 1

Product Type Specific: SIT phosphosite-specific antibody

Antibody Code: PN535

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y90

Protein UniProt: Q9Y3P8

Protein SigNET: Q9Y3P8

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human SIT sequence peptide Cat. No.: PE-04AEL99

Antibody Immunogen Sequence: VPL(pY)GNL(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues V87 to L93; In the Sit domain. This is the major in vivo phosphorylation site of SIT.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.