Product Name: SIT-pY90
Product Number: AB-PN535
Size: 25 µg      Price:89.00
      $US
Target Full Name: Signaling threshold-regulating transmembrane adapter 1

Target Alias: gp30/40; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SHP2 interacting transmembrane adaptor; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; signaling threshold regulating transmembrane adaptor 1; Signaling threshold-regulating transmembrane adapter 1; SIT; SIT1; suppression inducing transmembrane adaptor 1; Suppression-inducing transmembrane adapter 1

Product Type Specific: SIT phosphosite-specific antibody

Antibody Code: PN535

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y90

Protein UniProt: Q9Y3P8

Protein SigNET: Q9Y3P8

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human SIT sequence peptide Cat. No.: PE-04AEL99

Antibody Immunogen Sequence: VPL(pY)GNL(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues V87 to L93; In the Sit domain. This is the major in vivo phosphorylation site of SIT.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 19-21 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2, Jurkat, MCF7, and T98G cells as well as in sea star oocytes.

Related Product 1: SIT-pY90 blocking peptide

Related Product 2: SIT-pY95 phosphosite-specific antibody (Cat. No.: AB-PN536)

Scientific Background: SIT negatively regulates TCR (T-cell antigen receptor)-mediated signalling in T-cells. It is involved in positive selection of T-cells.
Figure 1. Epitope mapping of SIT-pY90 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to SIT-pY90.