Product Name: SHIP2-pY886
Product Number: AB-PN534
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2
Target Alias: 51C protein; Inositol polyphosphate 5-phosphatase; Inositol polyphosphate phosphatase-like 1; SH2-containing inositol 5-phosphatase 2; SHIP2
Product Type Specific: SHIP2 (INPPL1) phosphosite-specific antibody
Antibody Code: PN534
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y886
Protein UniProt: O15357
Protein SigNET: O15357
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: 51C protein; Inositol polyphosphate 5-phosphatase; Inositol polyphosphate phosphatase-like 1; SH2-containing inositol 5-phosphatase 2; SHIP2
Product Type Specific: SHIP2 (INPPL1) phosphosite-specific antibody
Antibody Code: PN534
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y886
Protein UniProt: O15357
Protein SigNET: O15357
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SHIP2 (INPPL1) sequence peptide Cat. No.: PE-04AES99
Antibody Immunogen Sequence: ERL(pY)EWI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E883 to I889; In the region between the Exo-endo_phos and SHAM_1 domains. This is one of the main in vivo phosphorylation sites in proteins.
Antibody Immunogen Sequence: ERL(pY)EWI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E883 to I889; In the region between the Exo-endo_phos and SHAM_1 domains. This is one of the main in vivo phosphorylation sites in proteins.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in HeLa cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-160 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in HeLa cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-160 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells, except phenylarsine oxide (PAO) increases 140 + 55 kDa proteins; No significant cross-reactivities detected in HepG2 cells.
Related Product 1: SHIP2-pY886 blocking peptide
Related Product 2: SHIP2-1 pan-specific antibody (Cat. No.: AB-NP045-1)
Related Product 3: SHIP2-2 pan-specific antibody (Cat. No.: AB-NP045-2)
Related Product 4: SHIP2-3 pan-specific antibody (Cat. No.: AB-NP045-3)
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells, except phenylarsine oxide (PAO) increases 140 + 55 kDa proteins; No significant cross-reactivities detected in HepG2 cells.
Related Product 1: SHIP2-pY886 blocking peptide
Related Product 2: SHIP2-1 pan-specific antibody (Cat. No.: AB-NP045-1)
Related Product 3: SHIP2-2 pan-specific antibody (Cat. No.: AB-NP045-2)
Related Product 4: SHIP2-3 pan-specific antibody (Cat. No.: AB-NP045-3)
Scientific Background: SHIP2 (INPPL1) is a phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, and negatively regulate the PI3K (phosphoinositide 3-kinase) pathways. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signalling or GLUT4 trafficking. It confers resistance to dietary obesity. It may also hydrolyze PtdIns(1,3,4,5)P4.
Figure 1. Epitope mapping of SHIP2-pY886 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to SHIP2-pY886.
© Kinexus Bioinformatics Corporation 2017