Product Name: PTRF-pY308
Product Number: AB-PN533
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Polymerase I and transcript release factor
Target Alias: Cavin-1; Leucine-zipper protein FKSG13
Product Type Specific: PTRF phosphosite-specific antibody
Antibody Code: PN533
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y308
Protein UniProt: Q6NZI2
Protein SigNET: Q6NZI2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PTRF sequence peptide Cat. No.: PE-04AEP95
Target Alias: Cavin-1; Leucine-zipper protein FKSG13
Product Type Specific: PTRF phosphosite-specific antibody
Antibody Code: PN533
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y308
Protein UniProt: Q6NZI2
Protein SigNET: Q6NZI2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PTRF sequence peptide Cat. No.: PE-04AEP95
Antibody Immunogen Sequence: HVV(pY)ARS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues H305 to S311
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Immunogen Description: Corresponds to amino acid residues H305 to S311
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-56 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and T98G cells.
Related Product 1: PTRF-pY308 blocking peptide
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and T98G cells.
Related Product 1: PTRF-pY308 blocking peptide
Scientific Background: PTRF plays an important role in caveolae formation and organization. It is required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
Figure 1. Epitope mapping of PTRF-pY308 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to PTRF-pY308.
© Kinexus Bioinformatics Corporation 2017