Product Name: PLCG2-pY759
Product Number: AB-PN531
Size: 25 µg      Price:89.00
      $US
Target Full Name: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2

Target Alias: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; Phosphoinositide phospholipase C-gamma-2; phospholipase C gamma 2; phospholipase C, gamma 2 (phosphatidylinositol-specific); phospholipase C, gamma 2 (phosphatidylyinositol-specific); Phospholipase C-gamma-2; Phospholipase C-IV; PLC-gamma-2; PLC-IV; PLCG2

Product Type Specific: Phospholipase phosphosite-specific antibody

Antibody Code: PN531

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y759

Protein UniProt: P16885

Protein SigNET: P16885
Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PLCG2 (PLC R) sequence peptide Cat. No.: PE-04ADH99

Antibody Immunogen Sequence: SRM(pY)VDP(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues S756 to P762; Near the end of the second SH2 domain in the protein. One of the major in vivo phosphorylation sites in PLCG2.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 140-160 kDa.

Related Product 1: PLCG2-pY759 blocking peptide

Related Product 2: PLCG1-pY783 phosphosite-specific antibody (Cat. No.: AB-PN530)

Scientific Background: PLCG2 mediates the production of the second messenger molecules diacylglycerol (DAG) and 1D-myo-inositol 1,4,5-trisphosphate (IP3) from the hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. It becomes activated in response to ligand-mediated activation of receptor-type receptor-tyrosine kinases and is calcium-activated.
Figure 1. Epitope mapping of PLCG2-pY759 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to PLCG2-pY759.