Product Name: PKM2-pY390
Product Number: AB-PN529
Size: 25 µg      Price:89.00
      $US
Target Full Name: Pyruvate kinase isozymes M1/M2

Target Alias: CTHBP; Cytosolic thyroid hormone-binding protein; KPYM; MGC3932; OIP-3; OIP3; Opa-interacting protein 3; p58; PK, muscle type; PK2; PK3; PKM; PKM2; Pyruvate kinase 2/3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; pyruvate kinase, muscle; TCB; THBP1; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tumour M2-PK

Product Type Specific: Pyruvate kinase phosphosite-specific antibody

Antibody Code: PN529

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y390

Protein UniProt: P14618

Protein SigNET: P14618
Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PKM2 sequence peptide Cat. No.: PE-04AHE95

Antibody Immunogen Sequence: AAI(pY)HLQ(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues A387 to Q393; At the end of the PK domain. A major phosphorylation site in PKM2.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 57-62 kDa.

Related Product 1: PKM2-pY390 blocking peptide

Scientific Background: PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, and generates ATP. It stimulates POU5F1-mediated transcriptional activation. It appears to plays a general role in caspase independent cell death of tumour cells. The ratio between its highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is thought to be important for tumour cell proliferation and survival.
Figure 1. Epitope mapping of PKM2-pY390 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to PKM2-pY390.