Product Name: PIK3R1-pY467
Product Number: AB-PN526
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
Target Alias: GRB1; p85; p85-ALPHA; P85A; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory, 1; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PIK3R1; PtdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-alpha
Product Type Specific: Lipid kinase phosphosite-specific antibody
Antibody Code: PN526
Antibody Target Type: Phosphosite-specific
Target Alias: GRB1; p85; p85-ALPHA; P85A; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory, 1; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PIK3R1; PtdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-alpha
Product Type Specific: Lipid kinase phosphosite-specific antibody
Antibody Code: PN526
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y467
Protein UniProt: P27986
Protein SigNET: P27986
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PIK3R1 sequence peptide Cat. No.: PE-04AHI99
Antibody Immunogen Sequence: DRL(pY)EEY(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D464 to Y470; In the region between the two SH2 domains in the C-terminus half of the protein. This is the major in vivo phosphorylation site in PI3KR1.
Protein UniProt: P27986
Protein SigNET: P27986
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PIK3R1 sequence peptide Cat. No.: PE-04AHI99
Antibody Immunogen Sequence: DRL(pY)EEY(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D464 to Y470; In the region between the two SH2 domains in the C-terminus half of the protein. This is the major in vivo phosphorylation site in PI3KR1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-85 kDa.
Antibody Specificity: Very high
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-85 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293 and Jurkat cells.
Related Product 1: PIK3R1-pY467 blocking peptide
Related Product 2: PIK3R1-pY580 phosphosite-specific antibody (Cat. No.: AB-PN527)
Related Product 1: PIK3R1-pY467 blocking peptide
Related Product 2: PIK3R1-pY580 phosphosite-specific antibody (Cat. No.: AB-PN527)
Scientific Background: PIK3R1 binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. It is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. It also mediates cellular signalling in response to various growth factors that bind to other receptor-tyrosine kinases, including EGF, IGF1, VEGFA and PDGF, as well as downstream proteins such as insulin-receptor substrate (IRS) proteins. It plays a role in ITGB2 signalling (PubMed:17626883, PubMed:19805105, PubMed:7518429), and modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress-and/or insulin-dependent manner during metabolic overloading in the liver to improve glucose tolerance (PubMed:20348923).
Figure 1. Epitope mapping of PIK3R1-pY467 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to PIK3R1-pY467.
© Kinexus Bioinformatics Corporation 2017