Product Name: PFN1-pY129
Product Number: AB-PN524
Size: 25 µg      Price:89.00
      $US
Target Full Name: Profilin 1

Target Alias: PROF1; Profilin I; Profilin-1

Product Type Specific: Profilin 1 phosphosite-specific antibody

Antibody Code: PN524

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y129

Protein UniProt: P07737

Protein SigNET: P07737

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PFN1 sequence peptide Cat. No.: PE-04ADJ75

Antibody Immunogen Sequence: KKC(pY)EMA(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues K126 to A132; In the end of the Profilin domain. This is the major in vivo phosphorylation site in Profilin 1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1.0 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 12-17 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.

Related Product 1: PFN1-pY129 blocking peptide

Scientific Background: At high concentrations, Profilin 1 binds actin and prevents its polymerization, whereas it enhances actin polymerization at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and diacylglycerol by sequestering PIP2. It inhibits androgen receptor (AR) and HTT aggregation, and the binding of G-actin that is essential for its inhibition of AR. Phosphorylation of PFN-1 Y129 induces interaction with VHL.
Figure 1. Epitope mapping of PFN1-pY129 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to PFN1-pY129.