Target Full Name: PDZ and LIM domain protein 5

Target Alias: ENH; ENH1; Enigma homolog; enigma-like LIM domain protein; Enigma-like PDZ and LIM domains protein; L9; LIM; PDLI5; PDLIM5; PDZ and LIM domain 5; PDZ and LIM domain protein 5

Product Type Specific: PDLIM5 (LIM) phosphosite-specific antibody

Antibody Code: PN522

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y251

Protein UniProt: Q96HC4

Protein SigNET: Q96HC4

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PDLIM5 (LIM) sequence peptide Cat. No.: PE-04ADC99

Antibody Immunogen Sequence: TEF(pY)HVP(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues T248 to P254; In the middle of the protein between the PDZ and first LIM domains. This is the mafor in vivo phosphorylation site of PDLIM5.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.