Target Full Name: Uncharacterized protein C9orf78

Target Alias: bA409K20.3; C9orf78; chromosome 9 open reading frame 78; CI078; HCA59; Hepatocellular carcinoma-associated antigen 59; HSPC220

Product Type Specific: HCA59 phosphosite-specific antibody

Antibody Code: PN518

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y147

Protein UniProt: Q9NZ63

Protein SigNET: Q9NZ63

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human HCA59 (Q9NZ63) sequence peptide Cat. No.: PE-04ACZ80

Antibody Immunogen Sequence: DCL(pY)ELP(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues D144 to P150; In the middle of the Hep_59 domain. This is the major in vivo phosphorylation site in HCA59.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.