Product Name: Gab1-pY406
Product Number: AB-PN516
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: GRB2-associated-binding protein 1
Target Alias: GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1
Product Type Specific: Gab1 phosphosite-specific antibody
Antibody Code: PN516
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y406
Protein UniProt: Q13480
Protein SigNET: Q13480
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Gab1 sequence peptide Cat. No.: PE-04ACW80
Target Alias: GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1
Product Type Specific: Gab1 phosphosite-specific antibody
Antibody Code: PN516
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y406
Protein UniProt: Q13480
Protein SigNET: Q13480
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Gab1 sequence peptide Cat. No.: PE-04ACW80
Antibody Immunogen Sequence: QDC(pY)DIP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q403 to P409; In the mid-region of the protein. This is the second major in vivo phosphorylation site of Gab1. Effect of phosphorylation is unclear.
Antibody Immunogen Description: Corresponds to amino acid residues Q403 to P409; In the mid-region of the protein. This is the second major in vivo phosphorylation site of Gab1. Effect of phosphorylation is unclear.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.3 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-strong immunoreactivity of a target-sized protein by Western blotting in HeLa cells (harvested in SDS-PAGE sample buffer).
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-strong immunoreactivity of a target-sized protein by Western blotting in HeLa cells (harvested in SDS-PAGE sample buffer).
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 75-100 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactivities detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells, and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE buffer.
Related Product 1: Gab1-pY406 blocking peptide
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactivities detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells, and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE buffer.
Related Product 1: Gab1-pY406 blocking peptide
Scientific Background: Gab1 is an adapter protein that plays a role in intracellular signalling cascades triggered by activated receptor-type kinases. It plays a role in FGFR1 signalling and is probably involved in signalling from the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR).
Figure 1. Epitope mapping of Gab1-pY406 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to Gab1-pY406.
© Kinexus Bioinformatics Corporation 2017