Product Name: ErbB2IP-pY1104
Product Number: AB-PN513
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Protein LAP2
Target Alias: Densin-180-like protein; erbb2 interacting protein; Erbb2-interacting protein; ErbB2IP; ERBIN; KIAA1225; LAP2; Protein LAP2
Product Type Specific: ErbB2IP phosphosite-specific antibody
Antibody Code: PN513
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1104
Protein UniProt: Q96RT1
Protein SigNET: Q96RT1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: Densin-180-like protein; erbb2 interacting protein; Erbb2-interacting protein; ErbB2IP; ERBIN; KIAA1225; LAP2; Protein LAP2
Product Type Specific: ErbB2IP phosphosite-specific antibody
Antibody Code: PN513
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1104
Protein UniProt: Q96RT1
Protein SigNET: Q96RT1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ErbB2IP (Erbin) sequence peptide Cat. No.: PE-04AER99
Antibody Immunogen Sequence: EGD(pY)LSY(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E1101 to Y1107; In the C-terminal quarter of the protein before the PDZ domain. The major in vivo phosphorylation site in ErbB2IP.
Antibody Immunogen Sequence: EGD(pY)LSY(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E1101 to Y1107; In the C-terminal quarter of the protein before the PDZ domain. The major in vivo phosphorylation site in ErbB2IP.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-weak immunoreactivity of a target-sized protein by Western blotting in HEK-293 and HeLa cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-weak immunoreactivity of a target-sized protein by Western blotting in HEK-293 and HeLa cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 170-180 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293 and HeLa cells, except for a very strong ~250 kDa cross-reactive protein in HeLa cells.
Related Product 1: ErbB2IP-pY1104 blocking peptide
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293 and HeLa cells, except for a very strong ~250 kDa cross-reactive protein in HeLa cells.
Related Product 1: ErbB2IP-pY1104 blocking peptide
Scientific Background: ErbB2IP acts as an adapter for the receptor ErbB2, in epithelia. By binding the unphosphorylated 'Y1248' of receptor ErbB2, it may contribute to stabilize this unphosphorylated state. It Inhibits NOD2-dependent NF-kappa-B signalling and proinflammatory cytokine secretion.
Figure 1. Epitope mapping of ERBB2IP-pY1104 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to ERBB2IP-pY1104.
© Kinexus Bioinformatics Corporation 2017