Target Full Name: Gamma-enolase

Target Alias: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2; enolase 2 (gamma, neuronal); Gamma-enolase; Neural enolase; neuron specific gamma enolase; Neuron-specific enolase; neurone-specific enolase; NSE

Product Type Specific: Enolase phosphosite-specific antibody

Antibody Code: PN512

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y25

Protein UniProt: P09104

Protein SigNET: P09104

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human ENO2 sequence peptide Cat. No.: PE-04ACT95

Antibody Immunogen Sequence: VDL(pY)TAK(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues V22 to K28; In the Enolase_N domain. This is one of the major in vivo phosphorylation sites in ENO2.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.