Target Full Name: Echinoderm microtubule-associated protein-like 4

Target Alias: C2orf2; DKFZp686P18118; echinoderm microtubule associated protein like 4; Echinoderm microtubule-associated protein-like 4; ELP120; EMAL4; EMAP-4; EMAPL4; EML4; FLJ10942; FLJ32318; Restrictedly overexpressed proliferation-associated protein; Ropp 120; ROPP120

Product Type Specific: EML4 phosphosite-specific antibody

Antibody Code: PN510

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y226

Protein UniProt: Q9HC35

Protein SigNET: Q9HC35

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human EML4 sequence peptide Cat. No.: PE-04AHG99

Antibody Immunogen Sequence: EGE(pY)IKM(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E223 to M229; In the N-terminal portion of EML4 near the HELP domain. This is the major in vivo phosphorylation site in EML4.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.