Product Name: Cas-L-pY166
Product Number: AB-PN505
Size: 25 µg      Price:89.00
      $US
Target Full Name: Enhancer of filamentation 1

Target Alias: NEDD9; Neural precursor cell expressed developmentally down-regulated protein 9

Product Type Specific: Cas-L phosphosite-specific antibody

Antibody Code: PN505

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y166

Protein UniProt: Q14511

Protein SigNET: Q14511

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Cas-L sequence peptide Cat. No.: PE-04AFA99
Antibody Immunogen Sequence: GYV(pY)EYP(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues G163 to P169; In the region between the SH3_9 and Serine_rich domains. This is the second major in vivo phosphorylation site in Cas-L.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in HeLa cells. Strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: Medium cross-reactive ~48 kDa protein detected in A431 and HeLa cells.

Related Product 1: Cas-L-pY166 blocking peptide

Scientific Background: CasL is a docking protein which plays a central coordinating role for tyrosine-kinase-based signalling related to cell adhesion. It functions in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. It may also participate in integrin beta-1 or B cell antigen receptor (BCR) mediated signalling in B- and T-cells.
Figure 1. Epitope mapping of Cas-L-pY166 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to Cas-L-pY166.