Target Full Name: Mixed-lineage protein-serine kinase 3; Mitogen-activated protein kinase kinase kinase 11

Target Alias: Kinase MLK3; M3K11; MAP3K11; MAPKKK11; MEKK11; Mitogen activated protein kinase kinase kinase 11; Mixed lineage kinase 3; MLK-3; SPRK; MGC17114; CCDS8107.1; ENSG00000173327

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK870

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T277+S281

Protein UniProt: Q16584

Protein SigNET: Q16584

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MLK3 sequence peptide Cat. No.: PE-04AQV65

Antibody Immunogen Sequence: WHK(pT)TQM(pS)AAGC

Antibody Immunogen Description: Corresponds to amino acid residues W274 to G280; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.