Product Name: YSK1-pT174
Product Number: AB-PK859
Size: 25 µg      Price:89.00
      $US
Target Full Name: Serine/threonine-protein kinase 25

Target Alias: DKFZp686J1430; SOK1; ST25; Ste20,oxidant stress response kinase-1; Ste20-like kinase; Sterile 20,oxidant stress-response kinase 1; STK25

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK859

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T174

Protein UniProt: O00506

Protein SigNET: O00506

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human YSK1 (STK25, SOK1) sequence peptide Cat. No.: PE-04ALL99

Antibody Immunogen Sequence: KRN(pT)FVG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues K171 to G177; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium-weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells with insulin-treatment.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-55 kDa.

Antibody Specificity: High to very high

Antibody Cross Reactivity: Strongly cross-reactive ~75-80 kDa protein detected in sea star oocytes and MCF7 cells as well as a ~21 kDa cross-reactive protein in MCF7 cells.

Related Product 1: YSK1-pT174 blocking peptide

Scientific Background: YSK1 (STK25) is a protein-serine/threonine kinase that is oxidant stress activated and hypothesized to play a role in the cellular response to environmental stress.YSK1 is specifically targeted to the Golgi apparatus where it is thought to function in the regulation of protein transport events, cell adhesion, and polarity complexes involved in cellular migration. Its C-terminal non-catalytic region inhibitsYSK1's phosphotransferase activity.
Figure 1. Epitope mapping of YSK1-pT174 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to YSK1-pT174.