Product Name: Tyk2-pY1054+pY1055
Product Number: AB-PK845
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Protein-tyrosine kinase 2 (Jak-related)
Target Alias: JTK1; Kinase Tyk2; Tyrosine kinase 2; CCDS12236.1; ENSG00000105397
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK845
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1054+Y1055
Protein UniProt: P29597
Protein SigNET: P29597
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Tyk2 sequence peptide Cat. No.: PE-04AHX99
Target Alias: JTK1; Kinase Tyk2; Tyrosine kinase 2; CCDS12236.1; ENSG00000105397
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK845
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1054+Y1055
Protein UniProt: P29597
Protein SigNET: P29597
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Tyk2 sequence peptide Cat. No.: PE-04AHX99
Antibody Immunogen Sequence: GHE(pY)(pY)RVR(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G1051 to R1058; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Immunogen Description: Corresponds to amino acid residues G1051 to R1058; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in A431 cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-140 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in A431 cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-140 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with JAK3 and JAK2, which feature similar phosphosite sequences. No significant cross-reactive proteins detected in A431 cells and HeLa cells.
Related Product 1: Tyk2-pY1054+pY1055 blocking peptide
Related Product 2: Tyk2-1 pan-specific antibody (Cat. No.: AB-NK181-3)
Related Product 3: Tyk2-3 pan-specific antibody (Cat. No.: AB-NK181-5)
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with JAK3 and JAK2, which feature similar phosphosite sequences. No significant cross-reactive proteins detected in A431 cells and HeLa cells.
Related Product 1: Tyk2-pY1054+pY1055 blocking peptide
Related Product 2: Tyk2-1 pan-specific antibody (Cat. No.: AB-NK181-3)
Related Product 3: Tyk2-3 pan-specific antibody (Cat. No.: AB-NK181-5)
Scientific Background: Tyk2 is a protein-tyrosine kinase of the TK group and JakA family. Phosphorylation of Y1054 and Y1055 increases phosphotransferase activity. Tyk2 mediates intracellular signal transduction from Type I interferon-alpha/beta receptor (IFN) signalling and phosphorylates the interferon-alpha/beta receptor alpha chain. Missense mutations in this gene have been found in colon and ovarian tumours.
Figure 1. Epitope mapping of TYK2-pY1054+pY1055 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to TYK2-pY1054+pY1055.
© Kinexus Bioinformatics Corporation 2017