Product Name: TRIM28-pY517
Product Number: AB-PK835
Size: 25 µg      Price:89.00
      $US
Target Full Name: Transcription intermediary factor 1-beta

Target Alias: KAP-1; KRIP-1; RING finger protein 96; RNF96; TIF1-beta; Tripartite motif-containing protein 28; TRIM28

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK835

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y517

Protein UniProt: Q13263

Protein SigNET: Q13263

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human TRIM28 (TIF1B) sequence peptide Cat. No.: PE-04APY99

Antibody Immunogen Sequence: TED(pY)NLI(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues T514 to I520; In the region between the zf-B_box and PHD domains. The major site of phosphorylation in TRIM28.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1.0 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-120 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: No significant cross-reactive proteins in A431, HeLa and MCF7 cells.

Related Product 1: TRIM28-pY517 blocking peptide

Related Product 2: TRIM28-pY458 phosphosite-specific antibody (Cat. No.: AB-PK834)

Scientific Background: TRIM28 (TIF1b)) is a protein-serine/threonine kinase of the Atypical group and TIF1 family. TIF family members are associated in retroviral restriction. TRIM28 contributes to EMT via E-cadherin and N-cadherin regulation in lung cancer cell lines.
Figure 1. Epitope mapping of TRIM28-pY517 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to TRIM28-pY517.