Product Name: TIE2-pY992
Product Number: AB-PK831
Size: 25 µg      Price:89.00
      $US
Target Full Name: Angiopoietin-1 receptor

Target Alias: Angiopoietin 1 receptor; CD202b antigen; HYK; P140 TEK; TEK; VMCM; VMCM1; OTTHUMP00000021167; TIE-2; hTIE2; ENSG00000120156

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK831

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y992

Protein UniProt: Q02763

Protein SigNET: Q02763

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human TIE2 (TEK) sequence peptide Cat. No.: PE-04ALI90

Antibody Immunogen Sequence: QEV(pY)VKK(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues Q989 to K995; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 135-150 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 cells.

Related Product 1: TIE2-pY992 blocking peptide

Related Product 2: TIE2-pY897 phosphosite-specific antibody (Cat. No.: AB-PK830)

Scientific Background: TIE2 (TEK) is a receptor protein-tyrosine kinase of the TK group and Tie family. It functions as a membrane receptor for ANGPT1, ANGPT2, and ANGPT4 and is involved in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion, actin cytoskeleton dynamics, and vascular quiescence. TIE2 expression is required for the normal development of the heart and blood vessels and for hematopoiesis post-natally. After birth, TIE2 expression can either promote or inhibit angiogenesis dependent upon the cellular context. In addition, TIE2 also has anti-inflammatory activity by preventing pro-inflammatory plasma proteins and leukocytes from leaking through the blood vessel endothelium. TIE2 directly phosphorylates DOK2, GRB7, GRB14, PIK3R1, Shc1, and TIE1 to mediate its effects. In mice, rats, and humans, TIE2 expression is almost exclusively in endothelial cells that line the vasculature. TIE2 has been demonstrated to be essential for the maintenance of hematopoeitic stem cells (HSCs) in a quiescent state in the bone marrow, which is required for the ongoing process of hematopoeisis. Phosphorylation at Y897 inhibits TIE2 phosphotransferase activity.
Figure 1. Epitope mapping of TIE2-pY992 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to TIE2-pY992.