Product Name: SRPK1-pS222
Product Number: AB-PK819
Size: 25 µg      Price:89.00
      $US
Target Full Name: Serine/arginine-rich protein-specific kinase 1; SRPK1a protein kinase

Target Alias: SFRS protein kinase 1; SFRSK1; SRPK1a protein kinase

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK819

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S222

Protein UniProt: Q96SB4

Protein SigNET: Q96SB4

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human SRPK1 sequence peptide Cat. No.: PE-04APR99
Antibody Immunogen Sequence: ILG(pS)DDD(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues I219 to D225; Near the N-terminus of the kinase.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-100 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: In HeLa cells, phenylarsine oxide (PAO) increases detection of 80 and 75 kDa proteins. No cross-reactivities observed in T98G cells.

Related Product 1: SRPK1-pS222 blocking peptide

Related Product 2: SRPK2-IKCD pan-specific antibody (Cat. No.: AB-NK296-1)

Related Product 3: SRPKSubtide - SRPK1 protein kinase substrate peptide

Scientific Background: SRPK1 (SFRSK1) is one of several protein kinases that can phosphorylate KinSub5RRLSP. It is a protein-serine/threonine kinase of the CMGC group and SRPK family. This kinase is moderate to highly expressed in most tested human tissues except apparently in the brain and spinal cord. SRPK1 is activated by phosphorylation at S51 and S555. In human cancer cells, it was observed that inactivation of SRPK1 induces cellular resistance to anticancer drugs such as cisplatin and bleomycin. SRPK1 phosphorylates serine/arginine-rich (SR) proteins, such as splicing factors ASF/SF2, SC35, and SRp55, in their arginine/serine-rich (RS) domains in vitro. Consequently, it is believed that SRPK1 plays a key role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors.
Figure 1. Dot blotting SRPK1-pS222 antibody with recombinant purified proteins.


Figure 2. Epitope mapping of SRPK1-pS222 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to SRPK1-pS222.