Product Name: SIK3-pT163
Product Number: AB-PK814
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Salt-inducible serine/threonine-protein kinase SIK3
Target Alias: FLJ12240; KIAA0999; L19; Salt-inducible kinase 3; Serine/threonine-protein kinase QSK; SIK family kinase 3; SIK3; SIK-3; L19; KIAA0999; ENSG00000160584
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK814
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T163
Protein UniProt: Q9Y2K2
Protein SigNET: Q9Y2K2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: FLJ12240; KIAA0999; L19; Salt-inducible kinase 3; Serine/threonine-protein kinase QSK; SIK family kinase 3; SIK3; SIK-3; L19; KIAA0999; ENSG00000160584
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK814
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T163
Protein UniProt: Q9Y2K2
Protein SigNET: Q9Y2K2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SIK3 (QSK) sequence peptide Cat. No.: PE-04APF99
Antibody Immunogen Sequence: LLK(pT)WCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L160 to G166; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Immunogen Sequence: LLK(pT)WCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L160 to G166; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of target-sized protein in maturing sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of target-sized protein in maturing sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 110-115 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in Jurkat cells.
Related Product 1: SIK3-pT163 blocking peptide
Related Product 2: SIK3-1 pan-specific antibody (Cat. No.: AB-NK250-1)
Related Product 3: SIK3-2 pan-specific antibody (Cat. No.: AB-NK250-2)
Related Product 4: SIK3-3 pan-specific antibody (Cat. No.: AB-NK250-3)
Related Product 5: SIK3-pT411 phosphosite-specific antibody (Cat. No.: AB-PK815)
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in Jurkat cells.
Related Product 1: SIK3-pT163 blocking peptide
Related Product 2: SIK3-1 pan-specific antibody (Cat. No.: AB-NK250-1)
Related Product 3: SIK3-2 pan-specific antibody (Cat. No.: AB-NK250-2)
Related Product 4: SIK3-3 pan-specific antibody (Cat. No.: AB-NK250-3)
Related Product 5: SIK3-pT411 phosphosite-specific antibody (Cat. No.: AB-PK815)
Scientific Background: SIK3 (QSK) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is a member of the salt-inducible kinase family. SIK3 is activated by phosphorylation on T163 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. It is a AMPK-family kinase that is ubiquitously expressed in the cytoplasm. In ovarian cancer, overexpression of SIK3 promotes G1/S cell cycle progression and cell proliferation and may therefore function as an oncoprotein.
Figure 1. Dot blotting SIK3-pT163 antibody with recombinant purified proteins.
Figure 2. Epitope mapping of SIK3-pT163 antibody with similar phosphopeptides on dot blots.
Figure 3. Identification of phosphosites related to SIK3-pT163.
© Kinexus Bioinformatics Corporation 2017