Target Full Name: Tyrosine-protein kinase SgK223; Homologue of rat pragma of Rnd2

Target Alias: FLJ00269; SG223; Sugen kinase 223

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK810

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y413

Protein UniProt: Q86YV5

Protein SigNET: Q86YV5

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Sgk223 sequence peptide Cat. No.: PE-04APO99

Antibody Immunogen Sequence: EPI(pY)AES(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E410 to S416; In the first third of the protein. This is the major in vivo site for phosphorylation of Sgk223.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.