Product Name: PKR1-pT446
Product Number: AB-PK777
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Double-stranded RNA-dependent protein-serine kinase; Interferon-induced, double-stranded RNA-activated protein kinase
Target Alias: ADRB2; E2AK2; EIF2aK; EIF2AK1; EIF2AK2; Eukaryotic translation initiation factor 2-alpha kinase 2; Interferon-inducible RNA-dependent protein kinase; P1,eIF-2A protein kinase; TIK; MGC126524; ENSG00000055332
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK777
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T446
Protein UniProt: P19525
Protein SigNET: P19525
Antibody Type: Polyclonal
Target Alias: ADRB2; E2AK2; EIF2aK; EIF2AK1; EIF2AK2; Eukaryotic translation initiation factor 2-alpha kinase 2; Interferon-inducible RNA-dependent protein kinase; P1,eIF-2A protein kinase; TIK; MGC126524; ENSG00000055332
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK777
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T446
Protein UniProt: P19525
Protein SigNET: P19525
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKR1 (PRKR; EIF2AK2) sequence peptide Cat. No.: PE-04APC99
Antibody Immunogen Sequence: GKR(pT)RSK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G443 to K449; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Immunogen Source: Human PKR1 (PRKR; EIF2AK2) sequence peptide Cat. No.: PE-04APC99
Antibody Immunogen Sequence: GKR(pT)RSK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G443 to K449; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-55 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-55 kDa.
Related Product 1: PKR1-pT446 blocking peptide
Related Product 2: PKR1-pT446 phospho-specific antibody (Cat. No.: AB-PK132)
Related Product 2: PKR1-pT446 phospho-specific antibody (Cat. No.: AB-PK132)
Scientific Background: PKR (PRKR, EIF2AK1) is a protein-serine/threonine kinase of the Other group and PEK family. It has an antiviral activity on various ranges of DNA and RNA viruses such as hepatitis C virus, hepatitis B virus, measles virus, and herpes simplex virus 1. It is activated by autophosphorylation after binding to dsRNA (generated during virus infection) in the presence of ATP. Besides double stranded RNA (dsRNA), heparin is a potent activator of the kinase. Binding to dsRNA is required for dimerization leading to autophosphorylation in the activation loop and stimulation of function. Activity is markedly stimulated by manganese ions. Autophosphorylation at S83, T88, T89, T90, Y101, Y162, S242, T255, T258, Y293 T446, T451, and S456 increase the phosphotransferase activity of EIF2AK2. Once activated, PKR can phosphorylate translation initiation factor EIF2S1, which in turn will inhibit protein synthesis. It can be inhibited by vaccinia virus protein E3, possibly via dsRNA sequestering. It has a role in p53-mediated responses to genotoxic stress. Since the protein has a crucial role for inflammation activation, it can be a potential therapeutic target to treat inflammation.
Figure 1. Identification of phosphosites related to PKR1-pT446.
© Kinexus Bioinformatics Corporation 2017