Product Name: PKCd-pY334
Product Number: AB-PK769
Size: 25 µg      Price:89.00
      $US
Target Full Name: Protein-serine kinase C delta

Target Alias: Kinase PKC-delta; KPCD; MAY1; MGC49908; nPKC-delta; PKC-delta; PRKCD; Protein kinase C, delta; CCDS2870.1; ENSG00000163932

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK769

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y334

Protein UniProt: Q05655

Protein SigNET: Q05655

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PKCd (PRKCD) sequence peptide Cat. No.: PE-04AOW95

Antibody Immunogen Sequence: SGT(pY)GKI(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues S331 to I337; In the region between the C1_1 and kinase catalytic domain. This is the major in vivo phosphorylation sites in PKCd.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1.0 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 78-85 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.

Related Product 1: PKCd-pY334 blocking peptide

Related Product 2: PKCd-pY313 phosphosite-specific antibody (Cat. No.: AB-PK768)

Related Product 3: PKCd-pT507 phosphosite-specific antibody (Cat. No.: AB-PK767)

Related Product 4: CREB1 (123-135) KinSub - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide

Related Product 5: PKCDSubtide - PKCd (PRKCD) protein kinase substrate peptide

Scientific Background: PKCd (PRKCD, PKC-delta) is a protein-serine/threonine kinase in the novel protein kinase C family. It is dependent on acidic phospholipids (e.g. phosphatidylserine) and diacylglycerol for full phosphotransferase activity, and does not require calcium. It is highly expressed and widely distributed in most tested human tissues, and is a major isoform of PKC expressed in hemopoietic cells. It is involved in promoting apoptosis, differentiation of cell types, cell growth, regulation of transcription factors, NFkB signalling induced by reactive oxygen species, and antigen presentation. PKCd is activated by phosphorylation at Y313, Y514, Y525, and S645. Phosphorylation of T507 increases phosphotransferase activity and induces degradation of PKC-delta. Phosphorylation of Y52, Y64, Y187, and Y567 inhibits phosphotransferase activity. Phosphatidylinositol is the most potent activator of PKCd. PKC delta is involved in fundamental cellular functions regulated by diacylglycerols, which is mimicked by phorbol esters. PKCd may be a tumour suppressor protein (TSP). PKCd negatively regulates cell proliferation in colon cell lines. It is also required for ErbB2-driven mammary gland tumourigenesis. Overexpression is seen in breast cancer and is associated with tamoxifen resistance. Reduced expression in MCF-7 cells is associated with a metastatic phenotype.
Figure 1. Dot blotting PKCd-pY334 antibody with recombinant purified proteins.


Figure 2. Epitope mapping of PKCd-pY334 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to PKCd-pY334.