Product Name: PDGFRa-pY768

Product Number: AB-PK759

Size:	25 µg		Price:	89.00
			$US

Target Full Name: Platelet-derived growth factor receptor kinase alpha

Target Alias: AI115593; Alpha platelet-derived growth factor receptor precursor; CD140a; Kinase PDGFR-alpha; MGC74795; PDGFR2; PDGFRa; PDGFR-alpha; PDGF-R-alpha; PGFRA; Platelet-derived growth factor, alpha-receptor; Rhe-PDGFRA; RHEPDGFRA; CD140A; MGC74795; CCDS3495.1; ENSG00000134853

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK759

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y768

Protein UniProt: P16234

Protein SigNET: P16234

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PDGFRa sequence peptide Cat. No.: PE-04AKU95

Antibody Immunogen Sequence: PAS(pY)KKK(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues P765 to K771; In the protein kinase catalytic domain near subdomain VI. This is the major in vivo phosphorylation sites in PDGFRa.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 115-135 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: Medium immunoreactivity on protein dot blots with recombinant human Flt3, which does not appear to feature a similar phosphosite.

Related Product 1: PDGFRa-pY768 blocking peptide

Related Product 2: PDGFRA-1 pan-specific antibody (Cat. No.: AB-NK242-1)

Related Product 3: PDGFRA-2 pan-specific antibody (Cat. No.: AB-NK242-2)

Related Product 4: PDGFRa-pS847+pY849 phosphosite-specific antibody (Cat. No.: AB-PK757)

Related Product 5: PDGFRa-pY762 phosphosite-specific antibody (Cat. No.: AB-PK758)

Related Product 6: PDGFRB-2 pan-specific antibody (Cat. No.: AB-NK243-1)

Scientific Background: PDGFRA is a receptor protein-tyrosine kinase in the TK family and PDGFR group. It acts as a cell surface receptor for platelet-derived growth factors PDGFA, PDGFB, and PDGFC. This kinase is highly expressed and widely distributed in most tested human tissues. PDGFRA can mediate cell survival, proliferation, embryonic development, chemotaxis, development of the mucosal layer of the gastrointestinal tract, intestinal villi development, wound healing, and platelet activation. The response of PDGFRA depends on whether it homodimerizes or heterodimerizes with PDGFRB. The heterodimer will phosphorylate PLCG1, PTPN11, and PIK3R1. Protein interactions are induced with phosphorylation of Y572 and Y574 (with Src), Y720 and Y754 (with SHP2), Y731 and Y742 (with PIK3R1), Y762 (with CDK6 & CrkL), and Y988 and Y1018 (with PLCg1). Aberrant expression of PDGFRA has been linked to developmental abnormalities and has been implicated in multiple disease states in humans. Widespread expression of PDGFRa occurs in renal cell types involved in fibrotic and sclerosing processes. PDGFRA induces fibrosis in the repair phase of inflammatory bowel disease and may be involved in the active inflammatory phase. PDGFRA is a known oncoprotein (OP). Cancer-related mutations in human tumours point to a gain of function of the protein kinase. The active form of the protein kinase normally acts to promote tumour cell proliferation. PDGFRA has also been linked with the development of atypical chronic myelogenous leukemia, glioblastoma multiforme (GM), melanomas, lung neuroendocrine carcinomas and gastrointestinal stromal cancers. PDGFRA is a known oncoprotein (OP). Cancer-related mutations in human tumours point to a gain of function of the protein kinase. The active form of the protein kinase normally acts to promote tumour cell proliferation. PDGFRA is linked to Gastrointestinal Stromal tumours (GANT, GIST), which are soft tissue sarcomas growing from the stomach, intestines, or rectum. A constitutively active PDGFRA can be induced with a V561D, N659K, D842V, or D842Y, mutation, or with the deletion of 842-845, or deletion of 845-848.

Figure 1. Dot blotting PDGFRa-pY768 antibody with recombinant purified proteins.

Figure 2. Epitope mapping of PDGFRa-pY768 antibody with similar phosphopeptides on dot blots.

Figure 3. Identification of phosphosites related to PDGFRa-pY768.