Product Name: PCTK2-pS180
Product Number: AB-PK756
Size: 25 µg      Price:89.00
      $US
Target Full Name: Cell division protein kinase 17; Protein-serine kinase PCTAIRE-2

Target Alias: CDK17; KPT2; PCTAIRE protein kinase 2; PCTAIRE-motif protein kinase 2; PCTK2; Serine/threonine-protein kinase PCTAIRE-2

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK756

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S180

Protein UniProt: Q00537

Protein SigNET: Q00537

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PCTK2 (PCTAIRE2, CDK17)) sequence peptide Cat. No.: PE-04AJM99

Antibody Immunogen Sequence: RRA(pS)LSE(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R177 to E183; Just before kinase catalytic domain. One of two major sites of phosphorylation of PCTK2.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-65 kDa.
Antibody Specificity: Very high

Related Product 1: PCTK2-pS180 blocking peptide

Related Product 2: PCTK2-ANT pan-specific antibody (Cat. No.: AB-NK285-1)

Scientific Background: PCTK2 (PCTAIRE2, CDK17) is a protein-serine/threonine kinase of the CMGC group and CDK family. It functions in terminally differentiated neurons. It is also identified to be associated with circulating phospholipid and sphingolipid concentrations. This kinase likely phosphorylates histone H1. Insertional mutagenesis studies in mice support a role for PCTK2 in mouse cancer oncogenesis.
Figure 1. Dot blotting PCTK2-pS180 antibody with recombinant purified proteins.


Figure 2. Epitope mapping of PCTK2-pS180 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to PCTK2-pS180.