Product Name: PCTK1-pY176
Product Number: AB-PK755
Size: 25 µg      Price:89.00
      $US
Target Full Name: Cell division protein kinase 16; Protein-serine kinase PCTAIRE-1

Target Alias: CDK16; FLJ16665; KPT1; PCTAIRE; PCTAIRE protein kinase 1; PCTAIRE-motif protein kinase 1; PCTGAIRE; PCTK1; Serine/threonine-protein kinase PCTAIRE1; ENSG00000102225

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK755

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y176

Protein UniProt: Q00536

Protein SigNET: Q00536

Antibody Type: Polyclonal

Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCTK1 (PCTAIRE1, CDK16) sequence peptide Cat. No.: PE-04AJL85

Antibody Immunogen Sequence: EGT(pY)ATV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E173 to V179; In the protein kinase catalytic domain in subdomain I. This is the major in vivo phosphorylation site in PCTK1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in HeLa cells that are treated for 30 minutes with phenylarsine oxide and vanadate. Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-64 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: In HeLa cells, phenylarsine oxide (PAO) increases detection of 100, 80, 75, 56, 48 and 27 kDa proteins.

Related Product 1: PCTK1-pY176 blocking peptide

Scientific Background: PCTK1 (PCTAIRE1, CDK16) is a protein-serine/threonine kinase of the CMGC group and CDK family. It has an important role in vesicle trafficking. Specifically neuronal release of GH1 is mediated by PCTK1. It is also involved in spermatogenesis, dendrite development, neuronal differentiation, and insulin secretion. BRSK2 mediated phosphorylation of PCTK1 can be inhibited with aPCTK1S12A mutation, which leads to fully inhibited effect on insulin secretion. The interaction of PCTK1 and CCNY can be inhibited with a S119 phosphorylation and PCTK1 phosphotransferase activity can be constitutively activated through the mutation S153A. Interaction with CCNY can be inhibited with a S153D mutation. Phosphorylation at S119 induces interaction with 14-3-3 gamma and 14-3-3 zeta. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
Figure 1. Dot blotting PCTK1-pY176 antibody with recombinant purified proteins.


Figure 2. Epitope mapping of PCTK1-pY176 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to PCTK1-pY176.