Target Full Name: Mitogen-activated protein-serine kinase p38 delta; Mitogen-activated protein kinase 13

Target Alias: MAP kinase p38 delta; MAPK13; Mitogen-activated protein kinase p38 delta; MK13; P38 MAPK-delta;p38delta; PRKM13; Stress-activated protein kinase-4; SAPK4; p38d MAPK; ENSG00000156711

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK742

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T180+Y182

Protein UniProt: O15264

Protein SigNET: O15264

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human p38d MAPK (MAPK13) sequence peptide Cat. No.: PE-04ADU85

Antibody Immunogen Sequence: AEM(pT)G(pY)(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues A177 to Y182; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.