Product Name: NuaK1-pT211
Product Number: AB-PK737
Size: 25 µg      Price:89.00
      $US
Target Full Name: NUAK family SNF1-like kinase 1; AMPK-related protein kinase 5

Target Alias: AMP-activated protein kinase family member 5; ARK5; KIAA053; KIAA0537; Kinase NuaK1; NUAK family, SNF1-like kinase, 1; NUAK2; Probable KIAA0537; ENSG00000074590

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK737

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T211

Protein UniProt: O60285

Protein SigNET: O60285

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human NuaK1 (ARK5)/Nuak2 sequence peptide Cat. No.: PE-04AKS99

Antibody Immunogen Sequence: FLQ(pT)FCG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues F208 to G214; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 70-76 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2 cells.

Related Product 1: NuaK1-pT211 blocking peptide

Scientific Background: NuaK1 is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It mediates cell proliferation, tumour progression, senescence, cell ploidy levels, and cell adhesion. It is Involved in tolerance to glucose starvation. It is activated by phosphorylation at T211 and S600. A T211A NuaK1 mutation can inhibit LKB1/LKB1-mediated activation and phosphorylation of NuaK1, while ablating induction of senescence. The I400K and L401K mutations can lead to interrupted NuaK1 complex with PPP1CB, and inhibiting regulation of PP1 activity. It suppresses Fas-induced apoptosis by phosphorylation of CASP6, and suppresses the activation of the caspase and the subsequent cleavage of CFLAR. It phosphorylates ATM. Ploidy by NuaK1 is mediated through the phosphorylation of S464 of LATS1. Cell adhesion control by NuaK1 is mediated via PP1 (protein phosphatase 1). Phosphorylation via PKB/Akt1 can be prevented through the S600A mutation.
Figure 1. Epitope mapping of NuaK1-pT211 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to NuaK1-pT211.